Quick Overview for Avidin Protein (AVD) (HRP) (ABIN964120)
抗原
See all Avidin (AVD) 蛋白蛋白类型
宿主
资源
应用范围
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标记
- This Avidin protein is labelled with HRP.
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Supplier Product No.
- a003-03
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Supplier
- Rockland
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原理
- Avidin Peroxidase Conjugated
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纯化方法
- Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above. Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single prec
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应用备注
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000
Western Blot Dilution: 1:10,000 - 1:40,000
ELISA Dilution: 1:20,000 - 1:200,000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Restore with deionized water (or equivalent) (in 1.0 mL)
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浓度
- 2.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Avidin Peroxidase Conju Expiration date is one (1) year from date of receipt.
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有效期
- 12 months
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- Avidin (AVD)
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别名
- Avidin
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背景
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Synonyms: HRP-Avidin conjugated, Horseradish peroxidase conjugated Avidin, AvD, HRP Avidin Biotin Complex, Avidin Peroxidase Conjugated, Avidin HRP Conjugated
Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the grea
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UniProt
- P02701
抗原
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